Today is 2009-01-07.

Reed and Muench Method Back

Calculation of ID50 based on Reed and Muench mathematical technique

Doses No. of infected No. of uninfected Accumulated Number Percentage of infected
Infected (A) Not infected (B) Total No. of tested(A+B)
105 10 0 31 0 31 31/31=100%
104 10 0 21 0 21 21/21=100%
103 8 2 11 2 13 11/13=85%
102 3 7 3 9 12 3/12=25%
101 0 10 0 19 19 0/19=0%

Calculation of the index

                   (% infected at dilution immediately above 50%) – 50%               
Index = _______________________________________________________________________________ 
             (% infected immediately above 50%) – (% infected immediately below 50%)

This is the application for calculating ID50, please prepare your data as show above, then fill out the form below

Please input your number of dilution factor (it is 10 in the table above)

Please input the series of dilution from low to high (e.g. 1, 2, 3, 4, 5 in the table above)

Please input numbers of positive treatments (infected in the above table) in the series of dilution from low to high (0, 3, 8, 10, 10 in the table above)

Please input numbers of negative treatments (uninfected the above table) in the series of dilution from low to high (10, 7, 2, 0, 0 in the table above)


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